rna | test runner for modern modules | Runtime Evironment library
kandi X-RAY | rna Summary
kandi X-RAY | rna Summary
We built RNA to be pluggable and to be interoperable with other build systems. A lot of esbuild and postcss plugins are distribuited as standalone packages in order to be reused outside the RNA opinionated ecosystem. We also designed a micro-sdk for esbuild plugin authors that handles transform pipelines and emits chunks or files.
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Currently covering the most popular Java, JavaScript and Python libraries. See a Sample of rna
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Trending Discussions on rna
QUESTION
I am trying to process bulk RNA-seq data using salmon through snakemake in the conda/mamba environment.
I am receiving the following error when running snakemake:
...ANSWER
Answered 2021-Jun-10 at 20:38I think the Snakefile is ok, SRR3350597_GSM2112330_RA_hip_3_Homo_sapiens_RNA-Seq_1.fastq.gz
is simply missing. See the ls
output of yours, that file is not in it.
QUESTION
I am using a cellranger mkref and faced with a strange python problem with GTF (custom gtf file):
...ANSWER
Answered 2021-May-26 at 21:50Python 3 treats strings of bytes as a different object from strings of characters. The distinction matters, since a given string of characters can be encoded to bytes in different ways. E.g. in UTF-8, ä
is the two bytes c3 a4
(in hex), while in ISO-8859-1 (Latin 1), it's just the single byte e4
.
Like the comment from @Theophrastus says, subprocess.check_output()
returns bytes, matching the low-level API. You need to decode it to characters based on what the expected encoding is. E.g.
QUESTION
I have a dataset that shows the human chromosomes with their length (which is here named "value") and their respective genes. Furthermore the genes are divided in 4 groups (gtype) which are RNA, prot-coding, pseudogene and rest. I want to plot the individual chromosomes on the y axis and on the x axis I want to have the density of the chromosomes (which would be "the number of genes per chromosome" divided by the length of the chromosome)with a geom_point,bar or col, then make a facet_wrap based on the gtype of the genes. So 4 plots with one plot only counting RNA genes divided by the value, one plot ony counting the prot-coding genes divided by value.
The plot just divides the total number of all genes by the individual values (it is however normal that chrM would be the biggest)
However I constantly fail at the x axis and I don't know how to get a plot that makes sense. What I have tried so far is a mix of sum(), count(), nrow() and group_by(). Often the x axis is just the total numbers of rows divided by the "value" or the results don't make sense.
...ANSWER
Answered 2021-Jun-03 at 22:19If I understand what you're doing correctly, your data looks a bit like this:
QUESTION
I am using a cellranger mkref and faced with a strange python problem with GTF (custome gtf):
...ANSWER
Answered 2021-Jun-01 at 20:27I encountered the same issue. The problem was in duplicated IDs in my GTF file. Removing those duplicates solved the issue. See the discussion on Cellranger GitHub: https://github.com/10XGenomics/cellranger/issues/125
QUESTION
I have a set of 270 RNA-seq samples, and I have already subsetted out their expected counts using the following code:
...ANSWER
Answered 2021-May-12 at 18:35If we want to name the list
, loop over the list
, extract the first element of 'expected_count' ('nm1') and use that to assign the names
of the list
QUESTION
I am trying to find names in one column and replace with it another column. Here is an example:
...ANSWER
Answered 2021-May-24 at 10:06Elya, you can simplify what you want to do. Most importantly, you do not need to loop over your data.
I assume your example data is representative for your dataset. In particular, the information in other
is separated by a semi-colon. In this case you can split the string by it and extract from your 3rd column the part required to add to the "gene_id" fragment in your 2nd column. Based on your comment I append a solution for multiple hits in the other column.
Note: the following names these columns "info", "gene_id", and "other". Make sure to adapt the variable naming.
data
QUESTION
I have raster files that have the same resolution and extent but differ in the number of NA. I want to unify the number of NA between all of them. Is it possible to do it by considering a cell as non-NA if it's not NA in all the raster files?
Here an example :
...ANSWER
Answered 2021-May-18 at 23:40It can be a bit confusing to use raster and terra together, so I will just use terra
(but you can do the same with raster
, using stack
in stead of c
and cellStats
in stead of global
.
Your example data
QUESTION
Code/Program:
...ANSWER
Answered 2021-Apr-29 at 16:59Your code is somewhat inefficient in the sense that it repeatedly splits the all the lines up. In the code below, this is only done once when they are first read in from the file. In addition, after reading they're transposed into columns of row since most of the processing is done with respect to what in each column.
QUESTION
I am trying to get the unique values of a column from a tab. The values are repeated and the file has 1,000+ lines, I just want to have the names of the values, not all, and the ones that are repeated. I'm working on my code, but when I do "RUN" it generates the separate and random letters of the values (see example in 'Output' below). I hope someone can help me find my mistake. Please and thank you very much!
Code:
...ANSWER
Answered 2021-Apr-27 at 14:11features
is just one string in one line of the file, not all the strings in that column.
Add each word to the unique_list
set in the loop, and print the set at the end.
QUESTION
Suppose I want to show in a barplot the gene expression results (logFC) based on RNA-seq and q-PCR analysis. My dataset looks like that:
...ANSWER
Answered 2021-Apr-22 at 07:19Following the linked answer, it seems quite natural how to extend it to your case. In the example below, I'm using some dummy data structured like the head()
data you gave, since the csv link gave me a 404.
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