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Trending Discussions on WGCNA
QUESTION
As far as I can find, there is only one tutorial about loading Seurat objects into WGCNA (https://ucdavis-bioinformatics-training.github.io/2019-single-cell-RNA-sequencing-Workshop-UCD_UCSF/scrnaseq_analysis/scRNA_Workshop-PART6.html). I am really new to programming so it's probably just my inexperience, but I am not sure how to load my Seurat object into a format that works with WGCNA's tutorials (https://horvath.genetics.ucla.edu/html/CoexpressionNetwork/Rpackages/WGCNA/Tutorials/).
Here is what I have tried thus far:
This tries to replicate datExpr and datTraits from part I.1:
...ANSWER
Answered 2021-Aug-22 at 23:27So doing as.matrix(datExpr)
right after datExpr <- t(sobjwgcnamat)[,VariableFeatures(sobjwgcna)]
worked. I had been trying it right before MEList = moduleEigengenes(datExpr, colors = moduleColors)
and that didn't work. Seems simple but order matters I guess.
QUESTION
I'm doing WGCNA on expression data and I performed a clustering using the hclust function:
...ANSWER
Answered 2021-May-19 at 16:49Here is a test using part of the iris
data set to see if the labels match:
QUESTION
I'm trying to take advantage of NumPy broadcasting and backend array computations to significantly speed up this function. Unfortunately, it doesn't scale so well so I'm hoping to greatly improve the performance of this. Right now the code isn't properly utilizing broadcasting for the computations.
I'm using WGCNA's bicor function as a gold standard as this is the fastest implementation I know of at the moment. The Python version outputs the same results as the R function.
...ANSWER
Answered 2020-Apr-07 at 23:52With a copy-n-paste of your X
:
QUESTION
powers = c(c(1:10), seq(from = 12, to=20, by=2));
While going through WGCNA i came across this code which i am not able to understand, can anybody explain me the meaning of that piece of code
...ANSWER
Answered 2020-Feb-18 at 08:43The code will create a vector of numbers stored in powers
.
Specifically: 1:10
creates the numbers 1 2 3 4 5 6 7 8 9 10
(can read as 1 through 10) and seq(from = 12, to = 20, by = 2)
creates a sequence of every other number from 12 to 20, i.e. 12 14 16 18 20
.
Powers will contain the following 15 numbers: 1 2 3 4 5 6 7 8 9 10 12 14 16 18 20
I am not familiar with the WGCNA
package or if powers
is an argument to a function, but this is what powers
contains.
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