lys | : hibiscus : Simple HTML templating for Python

I've been writing this code for my biology class. The idea is the user inputs teh DNA and the code outputs the respective mRNA and aminoacid sequence of it.It worked pretty much without any error but then i noticed it sometimes gives two outputs instead of one. (For example "Ile Met " instead of "Met") Also i know my code is a mess so can you please help by that too, which parts and how should i change?

So I'm struggling to solve a basic problem with some of the data I have and I can't seem to get round it. I have this data table

I can't for the life of me figure out how to convert this into a stacked bar chart that looks like this. I need the colours to represent the different Amino acids. Honestly I've been awake for 30 hours at this point so any help would be appreciated.

I've tried to convert to a long format however that still creates the same issue as before. When I use the default plot setting this is what I get

Here's some things I need help with.
But first of all, please let me pull up the code first.

Here's what I'm doing:

I am writing code that modifies a 3 letter sequence at all 3 positions separately by exchanging that position with one of the following A, T, C, or G.

I have been able to create 3 lists where the initial element has either the 1st, 2nd, or 3rd position modified to one of the other 3 different letters.

I have written a dictionary that encodes each key (amino acid in this case) and it's corresponding codon sequences (which would be the elements I am modifying). .

Now, I aim to check each modified list's elements against this dictionary, and see which dict key they correspond to. I wish see if changes in the initial element change the resulting key associated with it.

I am unable to figure out how to proceed; how can I get the corresponding key for the values of my modified lists?

Here is my code so far:

I have this code where lig_dec_residue is this nested dictionary which comes from big .dat files:

I have a long list of 190 variables, (they are destination routes). And I want to match, if any of these variables, exist in the array arr. (It is necessary for my job to have this many variables).

For some reason, the code below does not work. I'm not sure if it's because I have so many variables to check, perhaps the code is not efficient enough?

The route I'm specifically looking for in the array is "ORY-PSA", which is route72.

The only time my code works/returns true, is if I remove all the || checks, specifically type in the following code:

new to programming here.

In python I'm building up a script that allows one to enter a buffer pH and an amino acid's three letter code. The output should tell you the other amino acids that it should have electrostatic interactions with. The first iteration seems to work, however after that it stops returning any output no matter what parameters are entered. My guess is that somehow the boolean values are being replaced or something. Is there a way for each iteration to start completely fresh? I'd like the user to be able to continue entering parameters and have the output not be influenced at all by the previous iteration.

Here's the code (sorry if it's messy):

I have a file that looks like