crispr | The CRISPR static binary patcher | Parser library

I have just started using miniconda on windows and was creating an environment through a .yml file but got this error.

I am facing a problem regarding my python script. I am using Ubuntu on my machine as a second operating system and I have written a python code to output different information regarding some proteins. after giving me ouput for the first protein, it slows down way to much in the middle of the process for the second protein and at some point it wont respond anymore. when I type control+c I get this message :

I have a Dataframe with text in every cell. I want to iterate over the dataframe and the single characters of its cells and fill a list with either 0 for having a whitespace or 1 for having a character. I tried itertuples, iterrows and iteritems, but for all I can't access every single character of a string.

To give a little background, I'm trying to put together a pipeline to analyze deep sequencing results of in-silico predicted CRISPR off targets. I amplify a known sequence from the genome in 50 different places and each amplicon contains a predicted off target site where my original CRISPR guide could potentially bind. I input the two paired end NGS files into the program CRISPResso along with the amplicon sequence and off-target guide. The two paired end files, amplicon sequence, and gRNA are different for each of the 50 sites. I need to do this with multiple conditions, donors, and replicates so the numbers add up quick.

I created the snakemake workflow below:

Have a dna class where we add a few inputs into the big_strand (seems to work). Then take another input and subtracts it from the big_strand. This seems to work as well as long as I output within the subtraction function. However, after returning temp and going back to main, outputting simply gives a blank line(the grey line in the image is me highlighting the blank line). Any help is appreciated.

I have a csv file something like this

I'm currently using beautifulsoup to scrape a table on a site, this table includes links, I am then converting this table into a pandas dataframe and converting it to html using pandas 'to_html' option, this is all running in Django.

This is how I'm creating the table in Python:

I am viewing an image from url in an imageView and the code works fine with android lollipop and newer versions but not working with android Kitkat and older versions, it returns a blank image rather than the url image .. this is my code : HomeActivity.java

I am relatively new to tensorflow and I have attempted to adapt some code from a tutorial to process my own data.

The data can be found here: https://github.com/z12332/tensorflow-test-1/blob/master/export.csv

Keep in mind, that the dataset being fed consists of only columns 9 though 27 (with nan's converted to 0) and column 30 as the labels

here is the link to the tutorial code: https://github.com/llSourcell/How_to_use_Tensorflow_for_classification-LIVE/blob/master/demo.ipynb

I am able to get the program to run without an error message, but for some reason, it outputs 200 training steps of relatively random cost values. As and example, here are the first few steps: