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kandi X-RAY | Bioinformatics-Training Summary
kandi X-RAY | Bioinformatics-Training Summary
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QUESTION
As far as I can find, there is only one tutorial about loading Seurat objects into WGCNA (https://ucdavis-bioinformatics-training.github.io/2019-single-cell-RNA-sequencing-Workshop-UCD_UCSF/scrnaseq_analysis/scRNA_Workshop-PART6.html). I am really new to programming so it's probably just my inexperience, but I am not sure how to load my Seurat object into a format that works with WGCNA's tutorials (https://horvath.genetics.ucla.edu/html/CoexpressionNetwork/Rpackages/WGCNA/Tutorials/).
Here is what I have tried thus far:
This tries to replicate datExpr and datTraits from part I.1:
...ANSWER
Answered 2021-Aug-22 at 23:27So doing as.matrix(datExpr)
right after datExpr <- t(sobjwgcnamat)[,VariableFeatures(sobjwgcna)]
worked. I had been trying it right before MEList = moduleEigengenes(datExpr, colors = moduleColors)
and that didn't work. Seems simple but order matters I guess.
QUESTION
I am trying to add some headers to a txt file. Actually i have found a script already but I want to edit a part of it. Script (you can also find it here if you like: https://ucdavis-bioinformatics-training.github.io/2017-June-RNA-Seq-Workshop/thursday/counts.html):
...ANSWER
Answered 2020-Oct-29 at 15:59You can make use of cut
to get a range from a tab delimited line of words.
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