Porechop | adapter trimmer for Oxford Nanopore reads
kandi X-RAY | Porechop Summary
kandi X-RAY | Porechop Summary
While I'm happy Porechop has so many users, it has always been a bit klugey and a pain to maintain. I don't have the time to give it the attention it deserves, so I'm going to now officially declare Porechop as abandonware (though the unanswered issues and pull requests reveal that it already has been for some time). I've added a known issues section to the README to outline what I think is wrong with Porechop and how a reimplementation should look. I may someday (no promises though :stuck_out_tongue:) try to rewrite it from a blank canvas to address its faults.
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Top functions reviewed by kandi - BETA
Currently covering the most popular Java, JavaScript and Python libraries. See a Sample of Porechop
Porechop Key Features
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QUESTION
I am trying to process MinION cDNA amplicons using Porechop with Minimap2 and I am getting this error.
...ANSWER
Answered 2020-Sep-09 at 22:50First of all, you can always debug the problems like that specifying the flag --printshellcmds
. That would print all shell commands that Snakemake runs under the hood; you may try to run them manually and locate the problem.
As for why your rule doesn't produce any output, my guess is that samtools requires explicit filenames or -
to use stdin:
Samtools is designed to work on a stream. It regards an input file '-' as the standard input (stdin) and an output file '-' as the standard output (stdout). Several commands can thus be combined with Unix pipes. Samtools always output warning and error messages to the standard error output (stderr).
So try that:
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Vulnerabilities
No vulnerabilities reported
Install Porechop
If the last command complains about permissions, you may need to run it with sudo.
Install just for your user: python3 setup.py install --user If you get a strange "can't combine user with prefix" error, read this.
Install to a specific location: python3 setup.py install --prefix=$HOME/.local
Install with pip (local copy): pip3 install path/to/Porechop
Install with pip (from GitHub): pip3 install git+https://github.com/rrwick/Porechop.git
If you'd like to specify which compiler to use, set the CXX variable: export CXX=g++-6; python3 setup.py install
Porechop includes ez_setup.py for users who don't have setuptools installed, though that script is deprecated. So if you run into any installation problems, make sure setuptools is installed on your computer: pip3 install setuptools
By simply running make in Porechop's directory, you can compile the C++ components but not install an executable. The program can then be executed by directly calling the porechop-runner.py script.
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