barcoding | Lima - Demultiplex Barcoded PacBio Samples | Barcode Processing library
kandi X-RAY | barcoding Summary
kandi X-RAY | barcoding Summary
Lima - Demultiplex Barcoded PacBio Samples
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QUESTION
I'm trying to do is check first the item in listbox if the value in textbox is already in listbox.
...ANSWER
Answered 2022-Feb-23 at 10:41txtSS.Text = "a" In your editor set option strict to on to see what is wrong in code
QUESTION
I'm using the CATALYST package to analyze CYTOF data.
One of the commands requires a table in the format of a binary matrix.
I made the table in a .csv document and read it into R, which returned a dataframe-
...ANSWER
Answered 2021-Jan-11 at 02:19Starting with a frame:
QUESTION
I was wondering if anyone has experience in demultiplexing BCL files from Illumina with two different barcoding systems in one go?
we would like to know whether it is possible to use different, barcoded Tn5 adaptors during tagmentation and then use those Barcodes in combination with the barcodes on the sequencing primers to demultiplex the samples.
Has anyone has experience with this kind of analysis? Is it possible to only add the Tn5 sequence to the primer sequence before demultiplexing and use it in the BCL2fastq run?
thanks
Assa
...ANSWER
Answered 2020-Sep-14 at 09:47I'm not very well aware of Tn5 adaptors library but I will give it a shot.
In theory, it should be possible. bcl2fastq can demultiplex anything as long as you give it a proper samplesheet and a correct --use-bases-mask
argument.
If you need to demultiplex all samples with a combination of two barcode system, you will have to create a samplesheet with a line for each combination that can be found. You also need to know at which cycles the Tn5 adaptors are sequenced.
For example lets say my original samplesheet is like:
QUESTION
In books (e.g. "Barcodes for Mobile Devices", ISBN 978-0-521-88839-4), Papers (e.g. "Bar Codes May Have Poorer Error Rates Than Commonly Believed", DOI: 10.1373/clinchem.2010.153288) or websites information about the accuracy or error rates of barcodes are given.
The given numbers vary for e.g. Code39 from 1 error in 1.7 million, over 1 error in 3 million to 1 error in 4.5 million.
Where do these numbers come from and how can one calculate it (e.g. for Code39)?
In the definition of Code39 in ISO/IEC 16388:2007 I also couldn't find usefull information.
...ANSWER
Answered 2020-Jun-20 at 19:10Got to barcodefaq site you linked to and click on the Barcode type e.g. UPC and you will get a PDF that explains the methodology used. The article cited explains the error encountered as well as containing links to further informatiion.
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