RSEM | RSEM: accurate quantification of gene and isoform expression from RNA-Seq data | Genomics library
kandi X-RAY | RSEM Summary
kandi X-RAY | RSEM Summary
[Prior-enhanced RSEM (pRSEM)] uses complementary information (e.g. ChIP-seq data) to allocate RNA-seq multi-mapping fragments. We included pRSEM code in the subfolder pRSEM/ as well as in RSEM’s scripts rsem-prepare-reference and rsem-calculate-expression.
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QUESTION
In Snakemake, conda environments can be easily set up by defining rules as such conda: "envs/my_environment.yaml"
. This way, YAML files specify which packages to install prior to running the pipeline.
Some software requires a path to third-party-software, to execute specific commands.
An example of this is when generating a reference index with RSEM (example from GitHub page DeweyLab - RSEM):
...ANSWER
Answered 2020-Dec-15 at 06:34There are two ways that I've dealt with this.
1: Let Conda Handle PATHThat specific option (--star-path
) only needs to be specified if STAR is not on PATH. However, if STAR is included in your YAML for this rule, then Conda will place it on PATH as part of the environment activation, and so that option won't be needed. Same goes for --bowtie-path
. Hence, for such a rule the YAML might be something like:
QUESTION
I obtained a "XXX.cnt" in a newly created "XXX.stat" directory after an RSEM-1.3.3 analysis.
Shown below is the content of the XXX.cnt.
...ANSWER
Answered 2020-Sep-04 at 06:53The format and meanings of each field are described in "cnt_file_description.txt" under RSEM directory.
http://deweylab.github.io/RSEM/rsem-calculate-expression.html#OUTPUT
https://github.com/bli25broad/RSEM_tutorial
Here is the transcript.
QUESTION
I often find when adding rules to my workflow that I need to split large jobs up into batches. This means that my input/output files will branch out across temporary sets of batches for some rules before consolidating again into one input file for a later rule. For example:
...ANSWER
Answered 2020-Aug-27 at 07:22I hope I understood your problem correctly, if not, feel free to correct me:
So, you want to call the rule blast
for every "blast_input_{X}.fasta"
?
Then, the batch wildcard would need to be carried over into the output.
QUESTION
I have a data frame from TCGAbiolinks and need to narrow it down to just unnormalized data. I tried writing some sort of for loop that will return the rows where the tags variable in subset.gbmexp includes "unnormalized" but can't seem to get the code right. Something along the lines of:
...ANSWER
Answered 2020-Jul-30 at 21:05Assuming you want to select rows 1, 2, 3 and 6, which contain the character string "unnormalized" in the tags column, you could do:
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