LRSIM | 10x Genomics Reads Simulator | Genomics library
kandi X-RAY | LRSIM Summary
kandi X-RAY | LRSIM Summary
This package simulates whole genome sequencing using 10X Genomics Linked Read technology. We have attempted to realistically capture all of the relevant steps of the 10X protocol so that it can be used to faithfully evaluate linked read sequencing of different genomes, mutation rates, input libraries, and short read sequencing conditions in silico. We have tested the package with LongRanger, SuperNova, HapCUT2, fragScaff and ARCS to confirm that variant identifation, phasing, de novo assembly and scaffolding are supported. We also encourage users to use these simulations to aid in the development of novel algorithms. Please feel free to contact us if your pipelines require additional features.
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LRSIM Key Features
LRSIM Examples and Code Snippets
Usage: ./simulateLinkedReads.pl -r/-g -p [options]
Reference genome and variants:
-d INT Haplotypes to simulate [2]
-g STRING Haploid FASTAs separated by comma. Overrides -r and -d.
-1 INT 1 SNP per INT base pairs
The MIT License (MIT)
Copyright (c) 2016 Ruibang Luo
Permission is hereby granted, free of charge, to any person obtaining a copy
of this software and associated documentation files (the "Software"), to deal
in the Software without restr
git clone --recursive https://github.com/aquaskyline/LRSIM.git
cd LRSIM
sh make.sh
cd test
sh test.sh
Community Discussions
Trending Discussions on LRSIM
QUESTION
Getting the error at the below when trying to run a Perl script. The script is from this package: https://github.com/aquaskyline/LRSIM
...ANSWER
Answered 2019-Jun-21 at 12:21Around line 24093 of your strace
log make
says something about Clock skew detected
. Maybe the issue is related to the file system the script is running on (/mnt/fargen/experiments/...
)?
make
relies on accurate time stamps to determine which files need to be updated and to schedule its actions. If the time stamps are wrong, files might be built incorrectly or not at all.
The reason make
is involved at all is because behind the scenes Inline::C
extracts the embedded C code and creates an ad-hoc Perl module for it, including an XS file, Makefile.PL
, and the whole machinery. Then it invokes perl Makefile.PL && make && make install
, which is supposed to create a .so
file and copy it in a location where perl can load it (this .so
file is the loadable object mentioned in the error message).
Community Discussions, Code Snippets contain sources that include Stack Exchange Network
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