MISO | Isoforms model for RNA-Seq isoform quantitation | Genomics library
kandi X-RAY | MISO Summary
kandi X-RAY | MISO Summary
MISO (Mixture of Isoforms) is a probabilistic framework that quantitates the expression level of alternatively spliced genes from RNA-Seq data, and identifies differentially regulated isoforms or exons across samples. By modeling the generative process by which reads are produced from isoforms in RNA-Seq, the MISO model uses Bayesian inference to compute the probability that a read originated from a particular isoform. MISO uses the inferred assignment of reads to isoforms to quantitate the abundances of the underlying set of alternative mRNA isoforms. Confidence intervals over estimates can be obtained, which quantify the reliability of the estimates. Please see for MISO manual and documentation.
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QUESTION
I am trying to program an AVR ATTiny13A using a USBasp adapter (guloprog) and avrdude. Uploading the program and running it works fine the first time for a fresh ATTiny13A device, but re-uploading again fails. avrdude cannot see/access the device at all. avrdude behaves like the device is not connected.
...ANSWER
Answered 2022-Mar-06 at 19:19How to unbrick the AVR with a very slow clock divider setting
The problem is caused by writing CLKPR with a very slow clock divider setting (/128 or /256 with the 128kHz clock) in the program. After the program started, programming no longer works (regardless of the -B setting for avrdude).
Solution without HVSP (with pull-down resistor): Put a pull-down resistor between RESET and GND (10k worked for me). Now you can access the AVR again and program a different program, setting the clock divider to at most /64.
The pull-down resistor will keep the AVR in reset after power-up, not allowing the program to run, preventing the slow clock to be set. In order to run the new program you need to remove the pull-down resistor.
Alternative solution with HVSP
If you have a HVSP you can reset the CKSEL to 10 again to use the 9.6 MHz internal clock (and potentially set CKDIV8 to 0 again). This will allow you to access the device again.
Background
avrdude limits the slowest communication speed to a minimum of 500 Hz. This is hardcoded in the source, see https://github.com/avrdudes/avrdude/blob/5cbc9c37fc71c424e99bdcc00bb910fd581c2676/src/usbasp.c#L903
The device must be clocked at least four times faster than this speed. For 500 Hz communication speed this means that the AVR must run at 2000 Hz or higher. This means that the slowest clock-divider setting with the 128kHz clock which is compatible with avrdude is 128kHz/64 = 2000Hz (and this is already on the edge and may fail, but it worked well for me). To get the slowest avrdude communication speed (500 Hz) use the -B 2000 option, e.g.:
QUESTION
I am trying to connect to the ADXL345, which is on another board with GPIOs connected to it (PA5, PA6, PA7, PA12). Also, i am using PulseViewer from [sigrok][1] The Software shows, that the Sensor is NOT being enabled, yet it seems the functionality is correct. Is there something I'm missing?
...ANSWER
Answered 2021-Nov-22 at 08:27- SPI nCS lines are typicaly active-low, so PA12 must be set during board startup. In your code ADXL345 is always selected.
This GPIOA->BSRR |= GPIO_BSRR_BR_12; is reset (BR = Bit Reset, BS = Bit Set). Also, there is no need to use the |= operator, because BSRR is write-only register, designed to change port state without use of the read-modify-write sequence.
The
SPEbit inSPI_CR1regisger is not set, SPI is disabled and not transmitting anything.spi_txrxwould stuck in the secondSPI_SR_TXEwhile-loop.Not related to the question, but still -
spi_txrxlooks FIFO-oriened, yet STM32L0-series SPI have only shifter and DR register, and exchange must be implemented on the byte-by-byte basis, or with DMA. Perhaps this code would work for a 1- or 2-byte long transfer, and you'll even get a correct RX result if only last the byte is valuable. But generaly, it's a code smell.
QUESTION
so i have this page called screen1 its work fine and show the data
...ANSWER
Answered 2021-Nov-21 at 12:29The ListTile widgets themselves have an onTap event and do not require InkWell
QUESTION
if someone could explain why the getter method in this code is returning undefined, that would be appreciated. I figured it might be something to do with the 'this' context. Is the this.appetizers returning undefined because of the this context? When I call a method on the object and then that method calls the get method it fails, is this because the this context changes to the function object? I thought this could be the case but I have another code example that does this and it works fine.
...ANSWER
Answered 2021-Oct-29 at 23:26As mentioned in the comments, it's undefined. This is because even though you have a getter, it still sits under the _courses object:
QUESTION
I'm using the LilyGO TTGO T-beam with ESP32 to create a LoRa mesh network with RadioHead Library. I have met a problem that my mesh client is able to connect with the mesh server, but the server receives 0 bytes from the client. Are there any suggestions? Thanks. Current Outcome Picture
Settings:
...ANSWER
Answered 2021-Oct-29 at 02:32Ok, the problem solved, I need to use manager.available() to check the status and remove the manager.setModeRx().
QUESTION
I am working with CircuitPython Libraries on MicroPython using the Raspberry Pi Pico. I am using Wiznett 5500 (ethernet module) and Esp01 (wifi module). When I tried jsonplaceholder's api, Wiznett can get request in 4,5 seconds and ESP01 can get request in 1.6 second. When I tried my real api Wiznett 5500 can get request in 1 minute and Esp01 can get in 1.6 second. My api is really fast like microsecond, I don't understand why is wiznett getting data in 1 minute.
...ANSWER
Answered 2021-Oct-21 at 11:29You need to get more data not only {"exampleexa":888.8}. Add like 3 more lines.
QUESTION
I'm trying to use some previously developed sw from Github and ran across an interesting bit of sw coding.
He's using an Atmel ATtiny45 with digital I/O's on most of the PortB pins but PB3 is used as an analog (AtoD) input from an external potentiometer. In his code, he's got the following snippet:
...ANSWER
Answered 2021-Sep-30 at 19:11If we expand some of the preprocessor macros, your code becomes easier to explain:
QUESTION
I have build a prototype board with a STM8L, and I want it to be used and configured as a SPI slave. I am testing it with a raspberry pi as master.
I use the lib provided by ST called "STM8 Standard Peripherals Library" for this, but the documentation is very poor and doesn't expain how to do this...
I can send data from the Raspberry Pi with no issue and receive it on the STM8 but I can't send back any data to the raspberry from the STM8 on MISO.
Is anybody known how I can send back some data to the Raspberry Pi master? Where is my mistake?
Here is the main code:
...ANSWER
Answered 2021-Sep-26 at 19:14SPI requires the master to provide the clock. If you want the slave to send something - your master has to send some dummuy data to generate the clock for the slave.
QUESTION
I use an STM32F0 discovery board. I am trying to program an EEPROM to save an QR-code and display it on an LCD. Right now I am working on getting the STM32 to receive the data from the EEPROM, my code works if I short MISO and MOSI (I changed it a bit but not much), but when I try to get it to work with the EEPROM, it doesnt work.
My code below. Header file:
...ANSWER
Answered 2021-Sep-15 at 09:38So, after bashing my head against my table and my coworker stealing my oscilloscope, it worked, so i found out that the oscilloscope channels pulled my signal down bc i had the inputs in 50 Ohm mode and not 1MOhm. Switching that fixed it.
QUESTION
I am trying to use passing a choice of pins to the raspberry py when creating channels and want to change only the .P(value) when calling the method. For if I call the class in another class I currently have to import all libraries again with the way it is now. Below is code.
...ANSWER
Answered 2021-Aug-14 at 09:07I think you can define constants P0 to P7 in your module that defines createChannel, and then other files can import those constants from that module instead of getting them from MCP directly. Also, you can just specify a channel with an integer from 0 to 7.
I found some online documentation for adafruit_mcp3xxx.mcp3008. I think it means the channel names like MCP.P0 and MCP.P1 are really just integer values like 0 and 1, respectively.
The ADC chips’ input pins (AKA “channels”) are aliased in this library as integer variables whose names start with “P” (eg MCP3008.P0 is channel 0 on the MCP3008 chip). Each module that contains a driver class for a particular ADC chip has these aliases predefined accordingly. This is done for code readability and prevention of erroneous SPI commands.
You can make channel names available to users of your createChannel method by defining constants P0, P1 and so forth in the module that defines createChannel.
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