RSeQC | fork of RSeQC python RNAseq metrics suit of tools | Genomics library
kandi X-RAY | RSeQC Summary
kandi X-RAY | RSeQC Summary
This is a fork of original RSeQC package from sorceforge site I'm making drastic rearrangement to this package to make it easier to follow. I'm also making changes to the code base At this stage only read_dist (read_distribution) and bam_stats (bam_stat) modules have been incorporated and both now can be accessed from main executable scripts/rseqc. RSeQC package provides a number of useful modules that can comprehensively evaluate high throughput sequence data especially RNA-seq data. Some basic modules quickly inspect sequence quality, nucleotide composition bias, PCR bias and GC bias, while RNA-seq specific modules evaluate sequencing saturation, mapped reads distribution, coverage uniformity, strand specificity, transcript level RNA integrity etc.
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Top functions reviewed by kandi - BETA
- Process a gene model
- Return a list of all the cds
- Get a list of cdson objects
- Returns a list of Intergenic regions
- Calculate the strand - specificity of the gene model
- Convenience function for fetching sequences
- Processes the alignment file
- Check if there is one of the given ranges
- Return the length of the longest bed sequence in a sequence
- Reverse the reverse complement of a sequence
- Corrects the splicing bed file
- Calculate coverage coverage for each gene
- Show saturation rate for rpkm
- Calculate the inner distance between two sequences
- Calculates the Fickett - score of the DNA
- Extract genomic positions from a bed file
- Write data to rcode
- Convert BAM file into FASTQ format
- Returns the longest occurrence of a sequence
- Make a qual file plot
- Calculate coverage for each gene in a bigwig file
- Read the SeqRate plot
- Calculate deletion profile
- This function reads the NVC file
- Calculate the mismatch profile
- Calculate the kmer ratio
RSeQC Key Features
RSeQC Examples and Code Snippets
Community Discussions
Trending Discussions on RSeQC
QUESTION
I am trying to run a function called read_distribution.py in a Python package called RSeQC. However when I run the following command:
...ANSWER
Answered 2020-Dec-05 at 04:51I believe this package was written in Python 2, which didn't have you putting ()'s after print, in Python 3 this changed to have you put ()'s after print, You're using Python 3.
QUESTION
First off all, this could be a duplicate of Snakemake and pandas syntax. However, I'm still confused so I'd like to explain again.
In Snakemake I have loaded a sample table with several columns. One of the columns is called 'Read1', it contains sample specific read lengths. I would like to get this value for every sample separately as it may differ.
What I would expect to work is this:
...ANSWER
Answered 2019-Jan-08 at 15:59You could use lambda function
QUESTION
I start Snakemake on our qsub cluster like this:
...ANSWER
Answered 2018-Nov-30 at 21:28Here, rule_X
will use 12 ppn unlike other rules which use set default of 8.
QUESTION
The following snakemake code fails to produce multiqc output although it does for orther RSeQC tools including geneBody_coverage, junction_saturation, and read_distribution (removed here for clarity)
...ANSWER
Answered 2018-Nov-14 at 23:11The MultiQC documentation on RSeQC support shows that MultiQC does not support that particular tool (clipping_profile
), but it does support the others you mentioned.
Community Discussions, Code Snippets contain sources that include Stack Exchange Network
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Install RSeQC
You will need either sudo or virtualenvs (which is my preferred method). If you are you going to use sudo please prefix python setup.py install and pip install numpy with sudo. I haven't figured why, but numpy needs to be installed separately. It doesn't get pulled correctly from the dependencies list in setup.up.
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