CRISPResso | Software pipeline for the analysis of CRISPR-Cas9 genome | Genomics library
kandi X-RAY | CRISPResso Summary
kandi X-RAY | CRISPResso Summary
Software pipeline for the analysis of CRISPR-Cas9 genome editing outcomes from sequencing data
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CRISPResso Key Features
CRISPResso Examples and Code Snippets
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Trending Discussions on CRISPResso
QUESTION
To give a little background, I'm trying to put together a pipeline to analyze deep sequencing results of in-silico predicted CRISPR off targets. I amplify a known sequence from the genome in 50 different places and each amplicon contains a predicted off target site where my original CRISPR guide could potentially bind. I input the two paired end NGS files into the program CRISPResso along with the amplicon sequence and off-target guide. The two paired end files, amplicon sequence, and gRNA are different for each of the 50 sites. I need to do this with multiple conditions, donors, and replicates so the numbers add up quick.
I created the snakemake workflow below:
...ANSWER
Answered 2019-Dec-15 at 13:51The config file you show should have a space after the hyphen, i.e.:
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Install CRISPResso
You can use CRISPResso like any standard Python library. You will need to make sure that you have a development environment consisting of a Python distribution including header files, a compiler, pip, and git installed. Make sure that your pip, setuptools, and wheel are up to date. When using pip it is generally recommended to install packages in a virtual environment to avoid changes to the system.
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