scRNAseq | Bioconductor repository for the scRNAseq package | Genomics library
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Clone of the Bioconductor repository for the scRNAseq package.
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QUESTION
I have been trying to install scanpy package in anaconda using
...ANSWER
Answered 2021-Jan-13 at 13:41In your error log you can see
QUESTION
I am using anaconda to download an R package called scran. Although I have download it to my R library path, I received error. When I try to load my package in my R environment, it also failed. I am on macOS Catalina (10.15.4). I also try BiocManager::install(scran)
, but also failed.
scran (https://bioconductor.org/packages/release/bioc/html/scran.html)
Can someone give me a hand? Really appreciated! Please see the details below.
# I download scran using conda install -c Bioconda bioconductor-scran
ANSWER
Answered 2020-Apr-06 at 15:18I had your exact second error you are experiencing today, and think I have found a fix.
After doing some digging, I found my problem was with openblas not SCRAN. My fix was:
QUESTION
First of all, I’m new to rpy2 / jupyter so please don’t judge me if this isn’t the correct place to ask my question.
I am trying to set up an integrated workflow for data analysis using R and Python and I encounter the following error:
I am on Ubuntu 19.04. running a conda environment using Jupyter 1.0.0, Python 3.7.4, R 3.5.1, r-irkernel 1.0.2 and rpy2 3.1.0 and I installed the R-package Seurat through R.
When I create a Jupyter notebook using the R-kernel, I can load Seurat with library(Seurat)
just fine.
I can also use R code in python using rpy2 and the rmagic such as:
...ANSWER
Answered 2019-Nov-02 at 15:29The R package Seurat
is using an other R package called reticulate
, providing a bridge to Python from R.
Unfortunately, whenever rpy2
and reticulate
are involved R ends up being initialized twice, which results inevitably in a segfault. This is still an open bug at the time of writing. The issue tracking on the rpy2
side (a link to the reticulate
side of the tracking can be found there) is here:
https://bitbucket.org/rpy2/rpy2/issues/456/reticulate-rpy2-sharing-r-process
QUESTION
I'm following the example of Integrating CITEseq data with Deep Learning. The code worked until the third part of the example, where it is supposed to train the autoencoder. Since I'm new to keras models, I'm basically just copying and pasting the code, so I do not know how the one on the website is working and mine is not.
I've tried changing the fit funcion from
...ANSWER
Answered 2019-Oct-28 at 06:11Keras expects the first axis of your input data to be the number of samples. As you said, X_scRNAseq
shape is (36280, 8617)
and the shape of X_scProteomics
is (13, 8617)
. Keras expects the first axis to be the number of samples which isn't true in this case.
The solution, I believe, is to reshape both X_scRNAseq
and X_scProteomics
like so:
QUESTION
I am using the python
library:
https://github.com/ficusss/PyGMNormalize
For normalizing my datasets (scRNAseq
) and the last line in the library's file utils.py
:
https://github.com/ficusss/PyGMNormalize/blob/master/pygmnormalize/utils.py
uses too much of memory:
...ANSWER
Answered 2018-Sep-11 at 01:28I'm putting this as an answer since there's more than will fit in a comment, although it may not be complete. There's two suspicious things - first off percentile should run fine on a 20Gb matrix if your machine has 200Gb of ram available. That's a lot of memory, so start looking into what else might be using it. Start with top
- is there another process or is your python program using all of that?
The second suspicious thing is that the documentation for utils.percentile
doesn't match it's actual behavior. Here's the relevant bits from the code you've linked to:
QUESTION
I have a python
script that I am running on a SLURM
cluster for multiple input files:
ANSWER
Answered 2018-Sep-08 at 16:58You can get refined information by using srun
to start the python scripts:
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