tximport | summarize transcript-level estimates | Genomics library
kandi X-RAY | tximport Summary
kandi X-RAY | tximport Summary
Import and summarize transcript-level estimates for transcript- and gene-level analysis.
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QUESTION
I want to know if one can define a input rule that has dependencies on different wildcards.
To elaborate, I am running this Snakemake pipeline on different fastq files using qsub which submits each job to a different node:
- fastqc on original fastq - no downstream dependency on other jobs
- adapter/quality trimming to generate trimmed fastq
- fastqc_after on trimmed fastq (output from step 2) and no downstream dependency
- star-rsem pipeline on trimmed fastq (output from step 2 above)
- rsem and tximport (output from step 4)
- Run multiqc
MultiQC - https://multiqc.info/ - runs on the results folder which has results from fastqc, star, rsem, etc. However, because each job runs on a different node, sometimes Step 3 (fastqc and/or fastqc_after) is still running on the nodes while other steps finish running (Steps 2, 4 and 5) OR vice-versa.
Currently, I can create a MultiQc rule which waits on results from Steps 2, 4, 5 because they are linked to each other by input/output rules.
I have attached my pipeline as png to this post. Any suggestions would help.
What I need: I want to create a "collating" step where I want MultiQC to wait till all steps (from 1 to 5) finish. In other words, using my attached png as guide, I want to define multiple input rules for MultiQC that also wait on results from fastqc
Thanks in advance.
Note: Based on comments I received from 'colin' and 'bli' after my original post, I have shared the code for the different rules here.
Step 1 - fastqc
...ANSWER
Answered 2019-Jun-06 at 08:34If you want rule multiqc
to happen only after fastqc
completed, you can add the output of fastqc
to the input of multiqc
:
QUESTION
I have quantified gene expression by Salmon that gives me Ensembl transcripts, I converted Ensembl transcripts to gene symbol but for some genes I multiple transcripts; How I could collapse read counts to genes, I tried tximport
package but I found that too hard as my annotation is different.
ANSWER
Answered 2019-Apr-10 at 12:45You can use the package dplyr.
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