pysam | Python module for reading and manipulating SAM | Genomics library
kandi X-RAY | pysam Summary
kandi X-RAY | pysam Summary
Pysam is a Python module for reading and manipulating SAM/BAM/VCF/BCF files. It's a lightweight wrapper of the htslib C-API, the same one that powers samtools, bcftools, and tabix.
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pysam Key Features
pysam Examples and Code Snippets
from pysam import FastxFile
fasta_q_file = "INPUT.fasta"
out_filename = "OUTPUT_NAME.fasta"
size_size_trim = 50
with FastxFile(fasta_q_file) as fh, open(out_filename, mode='w') as fout:
for entry in fh:
sequence_id = entry.
import os
import sys
import pysam
from Bio import SeqIO, Seq, SeqRecord
def main(in_file):
out_file = "%s.fa" % os.path.splitext(in_file)[0]
with open('test_non_reverse.txt', 'w') as non_reverse:
with open(out_file, "w")
Community Discussions
Trending Discussions on pysam
QUESTION
I am running Snakemake with the --use-conda
option. Snakemake successfully creates the environment, which should include pysam. I am able to manually activate this created environment, and within it, run my script split_strands.py
, which imports the module pysam, with no problems. However, when running the Snakemake pipeline, I get the following error log:
ANSWER
Answered 2021-Mar-30 at 22:22Turns out that the newer version of snakemake 6.0.0+ must have some issue with this. I used snakemake 5.8.2 instead and things work just fine. Not sure exactly what's going on under the hood but seems identical to this issue: https://github.com/snakemake/snakemake/issues/883
QUESTION
I'm new to python and running the command:
pip install pysam
Which results in:
...ANSWER
Answered 2020-Feb-12 at 22:37if you have anaconda, try this:
conda install -c bioconda pysam
QUESTION
I have written a small and simple interface with Click, I get this error "Error: Got unexpected extra arguments" and I just can't figure out why this occurs?
Here is my setup.py:
...ANSWER
Answered 2020-Apr-06 at 07:11Your preprocess
function calls itself, resulting in the "unexpected extra arguments" error (see Click: "Got unexpected extra arguments" when passing string for a similar case). This is because its definition hides the one that you imported from .preprocessor
.
You should instead import the external preprocess
function using a different name, e.g.:
Community Discussions, Code Snippets contain sources that include Stack Exchange Network
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