nextflow | A DSL for data-driven computational pipelines | BPM library
kandi X-RAY | nextflow Summary
kandi X-RAY | nextflow Summary
Nextflow is a bioinformatics workflow manager that enables the development of portable and reproducible workflows. It supports deploying workflows on a variety of execution platforms including local, HPC schedulers, AWS Batch, Google Cloud Life Sciences, and Kubernetes. Additionally, it provides support for manage your workflow dependencies through built-in support for Conda, Docker, Singularity, and Modules.
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Currently covering the most popular Java, JavaScript and Python libraries. See a Sample of nextflow
nextflow Key Features
nextflow Examples and Code Snippets
FROM python:3.8.9-slim
LABEL
authors="phil.ewels@scilifelab.se,erik.danielsson@scilifelab.se" \
description="Docker image containing requirements for the nfcore tools"
# Do not pick up python packages from $HOME
ENV PYTHONNUSERSITE=1
nextflow.enable.dsl=2
params.publish_dir = System.getenv('TMPDIR') ?: './results'
params.publish_mode = 'copy'
check_params()
workflow {
// your awesome workflow here
}
def check_params() {
if( params.remove('help') ) {
input:
[from ] [attributes]
ch1 = Channel.of( 'hello', 'world' )
ch2 = Channel.of( 1, 3, 5, 7, 9 )
params.foo = false
params.bar = false
process test {
echo true
input:
val myval from ( params.
params.force = false
println("Force: ${params.force}")
process test {
echo true
when:
params.force
"""
echo "foo"
"""
}
nextflow run test.nf
N E X T F L O W ~ version 21.04.3
Launch
publishDir("${results_dir}/MY_PROC_RESULTS/", mode: "copy")
nextflow.enable.dsl=2
params.publish_dir = './results'
include { SUB_WORKFLOW_WF } from './SUB_WORKFLOW_WF_PROCS.nf'
workflow{
SUB_WORKFLOW_W
params.input_csv = '/file/location/here/file.csv'
input_csv = file( params.input_csv)
process unique {
input:
path input_csv
output:
path 'full_template.csv' into template
shell:
'''
awk 'BEGIN { FS=OFS=",
executor {
queueSize = 1
}
nextflow.enable.dsl=2
process test {
tag { "myval: ${myval}" }
input:
val myval
"""
sleep 1
"""
}
workflow {
myvals = Channel.of( 'A'..'Z' )
tes
nextflow.enable.dsl=2
process foo {
tag { sample }
input:
val sample
output:
path "${sample}.txt"
"""
if [ "${sample}" == "s1" ] ; then
(exit 1)
fi
if [ "${sample}" == "s2" ] ; then
proteins = Channel.fromPath( '/some/path/*.fa' ).collect()
executions = Channel.from(1, 2, 3, 4)
process blastThemAll {
tag { "job ${num}" }
input:
val num from executions
path query_file from proteins
"""
find . -name '*.f
Community Discussions
Trending Discussions on nextflow
QUESTION
How can I run a process with one instance for each pair of values in two lists, then collect the output of those instances along only one of the lists at a time?
For example, if you run this Nextflow script:
...ANSWER
Answered 2021-Jun-11 at 14:25One solution is to have your first process output a tuple which includes the 'number' as the first element, and then call groupTuple() to group together the files that share the same key:
QUESTION
I have the following (simplified) nextflow module. It has one process, which runs a multiple sequence alignment on a fasta file, and a workflow that runs this process (eventually it will run other processes too):
...ANSWER
Answered 2021-Apr-14 at 02:48Implicit workflow definitions are ignored when a script is imported as module. This means that your workflow script that can be used either as a library module or as an application script:
QUESTION
I am trying to enumerate files in a Channel to rename them before using collectFile
:
ANSWER
Answered 2021-Apr-14 at 01:50The migration notes say to use the join operator instead. If your inputs were lists, you could do something like:
QUESTION
I want to use Nextflow for creating pipelines of singularity components which will be executed by Slurm job scheduler. I created a simple job to run just a single task:
...ANSWER
Answered 2021-Mar-30 at 04:24Best to avoid calling Singularity manually, like in your script block. Assuming Singularity will be in your $PATH on every execution/compute node in your queue, then, all you'd need, for example, is:
In your nextflow.config
:
QUESTION
I have a nextflow script that creates a variable from a text file, and I need to pass the value of that variable to a command line order (which is a bioconda package). Those two processes happen inside the "script" part. I have tried to call the variable using the '$' symbol without any results, I think because using that symbol in the script part of a nextflow script is for calling variables defined in the input part.
To make myself clearer, here is a code sample of what I'm trying to achieve:
...ANSWER
Answered 2021-Mar-10 at 21:47declare "parameter" in the top 'params' section.
QUESTION
I'm new to nextflow/groovy/java and i'm running into some difficulty with a simple regular expression task.
I'm trying to alter the labels of some file pairs.
It is my understanding that fromFilePairs
returns a data structure of the form:
ANSWER
Answered 2021-Mar-08 at 14:49A closure can be provided to the fromfilepairs operator to implement a custom file pair grouping strategy. It takes a file and should return the grouping key. The example in the docs just groups the files by their file extensions:
QUESTION
I am attempting to merge x number of bam files produced via performing multiple alignments at once (on batches of y number of fastq files) into one single bam file in Nextflow.
So far I have the following when performing the alignment and sorting/indexing the resulting bam file:
...ANSWER
Answered 2021-Mar-04 at 15:53The simplest fix would probably to stop passing the static dirstring and runstring around via channels:
QUESTION
Using NextFlow (DSL=2), I would like to use each line of a file as a streaming input of my workflow.
...ANSWER
Answered 2021-Mar-03 at 02:38Your example code looks like an anti-pattern - it will try to create a new channel for each line in your input file. Instead, have a look at the splitting operators, especially the splitText operator:
QUESTION
Given the following nextflow.config
:
ANSWER
Answered 2021-Feb-25 at 01:59Overriding pipeline parameters can be done using Nextflow's command line interface by prefixing the parameter name with a double dash. For example, put the following in a file called 'test.nf':
QUESTION
How can I perform a try catch in nextflow?
I am currently writing a pipeline where it is possible that the bash command I am executing exits with an exitcode 1 under certain conditions. This brings my pipeline to a grinding halt. I would now like to use a try catch clause to define some alternative behavior in case this happens.
I have tried doing this in groovy fashion which does not seem to work:
...ANSWER
Answered 2021-Feb-11 at 23:29AFAIK there's no way to handle errors in your process definition using a try/catch block. Rather than trying to catch all of the scenarios that result in an exit status 1, could you better define those conditions and handle them before trying to execute your process? For example, if an empty FASTQ file (or a FASTQ file with an insufficient number of reads as required by your process) was supplied as input and this resulted in an exit status 1, a pre-processing command that filtered out those files could be useful here.
But if it's not possible to better define the condition(s) that your command produces exit status 1 or any non-zero exit status, you can ignore them like you have suggested by appending errorStrategy 'ignore'
to your process definition. Below is an example of how you could get the 'success' and 'failed' outputs, so they can be handled appropriately:
Community Discussions, Code Snippets contain sources that include Stack Exchange Network
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Nextflow can also be installed from Bioconda.
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