Roary | Rapid large-scale prokaryote pan genome analysis | Genomics library
kandi X-RAY | Roary Summary
kandi X-RAY | Roary Summary
Roary is a high speed stand alone pan genome pipeline, which takes annotated assemblies in GFF3 format (produced by Prokka) and calculates the pan genome. Using a standard desktop PC, it can analyse datasets with thousands of samples, something which is computationally infeasible with existing methods, without compromising the quality of the results. 128 samples can be analysed in under 1 hour using 1 GB of RAM and a single processor. To perform this analysis using existing methods would take weeks and hundreds of GB of RAM.
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QUESTION
I have a tab delimited binary matrix of bacterial strain names and genes, listed as present (1) or absent (0), which is output by ROARY (pangenome pipeline).
This is a mock version of the data:
...ANSWER
Answered 2021-Jun-08 at 19:55We can do a group by operation i.e. grouped by 'gene', check if all
the 'a', 'b' are found in the 'strain' where the 'pres_abs' value is 0 and to avoid getting the 1 values in pres_abs, create a second condition i.e. 'pres_abs' as 0
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