Microscope | seq analysis software suite for gene expression heatmaps | Genomics library

When I execute a python script (test2.py) from a running python script (test.py) I get the following error in test2.py:

I'm trying to get the edges of this object from a TEM(microscope) image and the problem is that the contact is low especially in the upper edge, I tried several things thresholding, contrast equalization... but I wasn't able to get the upper edge.

N.B: I'm trying to calculate the angle between the droplet and the tube I'm not sure if this is the best way to approach this problem.

The original image:

The Canny Edge detection I get:

the steps I got to get this result are:

1. Contrast enhancement
2. Thresholding
3. Gauss filter
4. Canny Edge detection

Code:

I want to make a product section with image and a name (title) and li(description) how to iterate through item 1,2,3 in products array such that it shows in li so I can make different objects for different products it doesn't seem to work when I do {{item.list}}

As the title suggests I am trying to update the axis units when using plt.imshow on .tif images captured from a microscope. Pixel --> microns. lets say 1 pixel is 0.5 microns.

I have made secondary Celsius axes in the past using callbacks, but didn't have luck with imshow. I briefly used imshow parameters origin and extent. It appears those are parameters for orientation. My apologies for providing code as I am writing this remote.

First a clarification that by "alias" I mean finder > right click on file > create alias, not .bash_profile stuff (are they the same? I don't know, I confess).

My scenario: I have a huge bunch of microscope scans, images taken at different positions, different focal depths, on different channels etc etc, all in one biiiiig folder.

Files are something along the line of TILE0123_FOCUS0123_CHANNEL0123.tiff. I would like to sort them in subfolders, but sometimesI need to group them by "same tile, same focus, all channels", sometimes "same focus, same channel, all tiles" and so on. My idea then was to leave the original files unsorted and create all the needed types of grouping with aliases.

The only previous post about python and osx alias paths I found is this one but to be honest it seems way more complicated than I hoped. So I tried selecting all images manually, right-click-create-alias them all, and then use my copying script. Notice that the copying script uses shutil.copy()

My problem: what gets copied is no longer an alias but just a txt file :(

I want to train some models to work with grayscale images, which e.g. is useful for microscope applications (Source). Therefore I want to train my model on graysale imagenet, using the pytorch grayscale conversion (torchvision.transforms.Grayscale), to convert the RGB imagenet to a grayscale imagenet. Internally pytorch rotates the color space from RGB to YPbPr as follows:

Y' is the grayscale channel then, so that Pb and Pr can be neglected after transformation. Actually pytorch even only calculates

First:
I am working on a dark-field microscopic tracking program. This program is old (2012), we got a new camera but this camera can´t be captured with a capture program. So I need to write a "capture program" for this camera with the SDK (Software development kit) from the company of the camera.

The tracking program is writen in C and GTK 2, so I would like to have the "capture program" in C and GTK 2 too, so I can just put the "capture program" in my tracking program. I can take a picture inside the "capture program" and show it as gtk_image_new_from_pixbuf.

Problem:
After being able to take pictures I found out that the dark-field microscope is not able to let you set the focus for the camera with your eyes. So now I need to capture a "live image", video or how ever you want to call it.
My idea was to use a timer so the camera will take a picture every second and display it as an image so the program has to delete the old image and show the new one. But that doesn´t happen I am pretty sure that there is no problem with the camera, because it shows the first image. My guess is that the pixbuf isn´t cleared and that causes that there is no new image shown.
The "capture program" doesn´t have to stay as a C/Gtk2 program but as I am not a computer scientist I just have no idea how to get the images into the tracking program if they are different.

Code:
I know that gdk_pixbuf_new_from_data has a starde of width*1 and not width*3 that is no problem.

I have a UIFrame with a getConfiguration() function which returns a TagGroup. But when compiling the script (with the TEM attatched) I get the error No match for =( TagGroup, RealNumber ) telling me that the returned value of the function is a real number. But as shown in the code below, the getConfiguration() function clearly has the return type of a TagGroup.

I want to create table which displays the detail of patient from its patient ID but my data is not displaying in the table i am new to java can anyone help me to solve this problem pleas give me solution to this problem there is no error or warning showing while compiling and running the program String tbcol[] is to come first and then below that Data from database should be displayed here is my program also is there any mistake in my SELECT Query