rBLAST | Interface for the Basic Local Alignment Search Tool | Genomics library
kandi X-RAY | rBLAST Summary
kandi X-RAY | rBLAST Summary
Interfaces the Basic Local Alignment Search Tool (BLAST) to search genetic sequence data bases with the Bioconductor infrastructure. This includes interfaces to blastn, blastp, blastx, and makeblastdb. The BLAST software needs to be downloaded and installed separately.
Support
Quality
Security
License
Reuse
Top functions reviewed by kandi - BETA
Currently covering the most popular Java, JavaScript and Python libraries. See a Sample of rBLAST
rBLAST Key Features
rBLAST Examples and Code Snippets
Community Discussions
Trending Discussions on rBLAST
QUESTION
I'm trying to use rBlast
for protein sequences but somehow it doesn't work. It works fine for nucleotide sequences but for proteins it just doesn't return any match (I used a sequence from the searched dataset, so there can't be no match). In the description it stands "This includes interfaces to blastn, blastp, blastx..." but in the help file in R studio it says "Description Execute blastn from blast+". Did anybody run rBlast
for proteins?
Here's what I ran:
...ANSWER
Answered 2021-Sep-30 at 06:10Tried to reproduce the error but everything worked as expected on my system (macOS BigSur 11.6 / R v4.1.1 / Rstudio v1.4.1717).
Given your blastn was successful, perhaps you are combining multiple fasta files for your protein fasta reference database? If that's the case, try concatenating them together and use the path to the file instead of an R object ("fa") when making your blastdb. Or perhaps:
Community Discussions, Code Snippets contain sources that include Stack Exchange Network
Vulnerabilities
No vulnerabilities reported
Install rBLAST
Install the package devtools.
Install the Bioconductor package Biostrings either using devtools::install_bioc("Biostrings") or directly from [Bioconductor](http://www.bioconductor.org/install/).
Install rBlast from GitHub using devtools::install_github("mhahsler/rBLAST").
Install the BLAST software by following the instructions found in ? blast
Support
Reuse Trending Solutions
Find, review, and download reusable Libraries, Code Snippets, Cloud APIs from over 650 million Knowledge Items
Find more librariesStay Updated
Subscribe to our newsletter for trending solutions and developer bootcamps
Share this Page