shovill | ️ Assemble bacterial isolate genomes from Illumina | Genomics library
kandi X-RAY | shovill Summary
kandi X-RAY | shovill Summary
The SPAdes genome assembler has become the de facto standard de novo genome assembler for Illumina whole genome sequencing data of bacteria and other small microbes. SPAdes was a major improvement over previous assemblers like Velvet, but some of its components can be slow and it traditionally did not handle overlapping paired-end reads well. Shovill is a pipeline which uses SPAdes at its core, but alters the steps before and after the primary assembly step to get similar results in less time. Shovill also supports other assemblers like SKESA, Velvet and Megahit, so you can take advantage of the pre- and post-processing the Shovill provides with those too. :warning: Shovill is for isolate data only, primarily small haploid organisms. It will NOT work on metagenomes or larger genomes. Please use Megahit directly instead.
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QUESTION
I am wondering how to deal with empty output files in downstream rules. An assembly of a short read fastq data with SHOVILL can fail and produce a 0-byte contigs.fa
If genome annotation with PROKKA is run on a 0-byte file it returns an error:
...ANSWER
Answered 2021-Aug-27 at 16:00I can think of two approaches, neither is perfect.
The first is basically what you did: use bash to work around. However, I would suggest the -s
file test operator. This way, you still get notified of a genuine error from prokka:
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